7. Tgam – wheat roots interaction
Four wheat seeds were placed on PDA at 1.5 cm of distance from the centre of the Petri dish. Plates were sealed with tape and incubated for 24 h at 22℃. After 24 h, 1 cm2 agar plug cut out at the border of 1-week-old colony of Tgam was placed in the centre of the plates containing wheat seedlings, as well as in PDA plates without plants as controls. Plates were sealed with tape and incubated for 3 days in the same conditions described above. Wheat roots colonized by Tgam were collected, frozen in liquid N2 and stored at -80℃ until RNA extraction. Mycelium from Tgam PDA control plates was collected and stored until use. Three biological replicates were included for each condition.