Figure legends
Figure 1: (A) Differences in bacterial alpha diversity
(Shannon diversity, Richness, Evenness Chao and Fishers Alpha) between
H1 & H2 heights. (B) Differences in bacterial alpha diversity
(Shannon diversity, Richness, Evenness, Chao and Fishers Alpha at all
heights (H1, H2, H3 & H4). (C) Rarefraction curvewith error
bars showing confidence intervals, for all four groups of samples based
on OTUs detected using a similarity threshold of 97% (0.97) and phylum
and genus levels.
Figure 2: Supervised PCA and Canonical Correspondence Analysis
(CCA) displaying the compositional distribution of the fecal microbiota
at altitudes H1 and H2, comparing 16s rRNA and WGS results:(Ai) 16srRNA PCA and (Aii) CCA analysis, (Bi)WGS PCA and (Bii) CCA analysis. PERMDISP 2 Analysis indicating
differences in global microbial community composition comparison at two
heights H1 and H2: (Aiii) 16srRNA and (Biii) WGS.
PERMDISP 2 Analysis indicating differences in global microbial community
composition comparison at all heights H1, H2, H3, and H4 (C)PERMDISP 2, (D) CCA analysis .
Figure 3: (A) Differentially abundant &
significant taxa in WGS data at phylum level (evaluated using T test at
a p-value cut off of 0.05) at four heights H1, H2, H3 and H4.(B) Odds ratio at 95 % CI &at a p-value cut off of 0.05 at
Heights H2 vs H1. (C) Differentially abundant & significant
taxa in 16SrRNA data at Genus level using Wilcoxon rank test(D) Q-Q plots of p values for the association between the
relative abundance and presence/absence of phyla.
Figure 4: (A) Differentially abundant & significant taxa in WGS
data at Genus level (evaluated using T test at a p-value cut off of
0.05) at four heights H1, H2, H3 and H4. (B) Odds ratio at 95 % CI &at
a p-value cut off of 0.05 at Heights H2 vs H1. (C) Differentially
abundant & significant taxa in 16SrRNA data at Genus level using
Wilcoxon rank test (D) Q-Q plots of p values for the association between
the relative abundance and presence/absence of Genus.
Figure 5: FastTree uses, generalised time-reversible (GTR) models
of nucleotide evolution in addition to the CAT Model (evolutionary rate
heterogeneity) approximation, and finally
for estimating the
reliability of each split in the tree using the Shimodaira-Hasegawa
test, at 1000 resamples.
Figure 6: Individual Significant Bacterial Profiles at all Time
points.
Figure 7: (A) Functional characteristics of gut
microbiomes of individuals at different altitudes (H2, H3 & H4) as
compare with H1. (B) Alteration in metabolic pathways at H1 and
H2 predicted by 16sRNA.