Specimen detection
All of the seven most common respiratory viruses were tested in each
child, among which PIV l, PIV 2, PIV 3, RSV and ADV were detected by
direct immunofluorescence assay using the D³ Ultra DFA Respiratory Virus
Screening and ID Kit (Diagnostic Hybrids, Inc., USA), and Flu A and F1u
B were detected by colloidal gold method using the Influenza A&B
Antigen detection Kit (Wondfo Biotech Co., Ltd, China). All the batch
numbers of the reagents are within the period of validity, and the
standard reference substance of the reagent was taken with each test.