Comparison of species composition between morphology, bulk DNA and eDNA datasets
The number of unique and shared species for the morphological, bulk and ethanol datasets was evaluated using the unrarified datasets from the four samples that had been identified morphologically and using the UpsetR package(Conway, Lex, & Gehlenborg, 2017). For the morphological dataset, only specimens identified up to species level were included in the dataset (juvenile identifications to genus, order or phylum level were removed from the dataset). For each of the five primer sets, the total number of species found for each of the four samples was determined and visualized in barplots using R.