Intralymphatic immunotherapy
The patients were randomized into three groups receiving three doses at
four-week intervals of 0.1 ml of birch pollen allergen on aluminum
hydroxide (10,000 SQ-U/ml; ALK-Abelló, Hørsholm, Denmark) and/or 0.1 ml
of 5-grass pollen allergen on aluminum hydroxide (10,000 SQ-U/ml;
ALK-Abelló, Hørsholm, Denmark. 5-grass is a mix of equal amounts of SQ-U
of Alopecurus pratensis (meadow foxtale), Dactylis
glomerata (cocks’s foot), Festuca pratensis (meadow fescue),Lolium perenne (English ryegrass), and Phleum pratense(timothy). Each allergen dose was 1.000 SQ-U. A diluent from ALK was
used as placebo. Thus the received two injections, one in each groin on
three occasions. Patients were randomized in blocks of six, facilitated
by Forum Östergötland. An unblinded nurse prepared and marked each
syringe with a label providing randomization number, injection number
and injection site. ILIT was administered by three clinicians (LA, PR,
and UN). Ultrasound-led technique was used whereby a lymph node was
punctured with a 27G (0.4 x 40 mm) needle. Histamine-1 blocker
desloratadine tablet 5 mg was given 15 minutes prior to the injections.