Conjunctival allergen provocation tests, IgE, IgG4 and skin
prick tests
CAPT with timothy were performed before ILIT and the first year post
ILIT, and showed a higher tolerance threshold in patients receiving ILIT
with birch and 5-grass (p<0.05) and 5-grass and placebo
(p<0.05), but not significant in patients who received ILIT
with birch and placebo (p=0.19, see Table S1 in the Supplementary
information). The IgE levels to birch decreased from baseline to three
years post ILIT from 25.45 to 17.71 kU/L (p<0.01 in the group
treated with birch and placebo; in the group treated with 5-grass and
placebo levels went from 33.18 to 24.42 (p<0.01). The levels
of IgE to timothy decreased after ILIT with birch and placebo from 16.60
to 11.00 kU/L (p<0.05); similar, but non-significant,
differences were determined after ILIT with 5-grass and placebo. IgE
levels to both birch and timothy decreased slightly after ILIT with both
birch and 5-grass (see Table S1 in the Supplementary information).
Levels of IgG4 antibodies to birch and timothy remained unchanged in all
three treatment groups, except for IgG4 levels to timothy, which
increased from in mean 0.36 to 0.44 mg/L (p<0.05) after ILIT
with birch and 5-grass (see Table S1 in the Supplementary information).
Skin prick test for reactivity to birch and timothy allergens remained
unchanged during the study period (see Table S1 in the Supplementary
information).