Secondary outcome measures
Effects on quality of life were measured using the rhinoconjunctivitis quality of life questionnaire (RQLQ) 22 recorded as RTSS and MS, see above. Skin prick test reactions (Soluprick SQ Birch and Timothy, ALK-Abelló), allergen-specific IgE and allergen-specific IgG4 levels were analyzed (ImmunoCAP ThermoFisher, Uppsala, Sweden) before ILIT and in the fall the following three years. Conjunctival allergen provocation tests (CAPT) 23 were performed with timothy (Aquagen SQ Timothy, ALK-Abelló) before treatment and after the first pollen season after treatment. Due to lack of extract from the company planned CAPT were not performed after the third pollen season (Table 2).