Visit 1: Pre-ILIT, pre-season. Visit 2: pre-ILIT, post-season. Visit 3; 7-35d post visit 2. Visit 4: 28-42d post visit 3. Visit 5: 28-42d post visit 4. Visit 6: Fall year 1. Visit 7: Fall year 2. Visit 8: Fall year 3.
1 Haematology: leukocytes, leukocyte differentiation (neutrophil, eosinophils, basophils, lymphocytes) haemoglobin and platelets at visits 1, 2, and 6. Coagulation blood tests at visits 1 and 2 (PK-INR and APTT).
2 Total IgG and subsets (lgG1-G4), total IgE, allergen-specific IgE and IgG4, and other immunological tests.
3 RTSS, RQLQ and MS were measured after the birch pollen season (approx. Jun 1st) and after the grass pollen season (approx. Aug 1st)
4 The conjunctival challenge tests (CAPT) with timothy were performed according to the EAACI guidelines 23. Due to lack of extract from the company planned CAPT were not performed after the third pollen season
5 The diary had space for description of the adverse events (AEs) since the last visit.
6 Two to five days after visit 3–5, telephone contact was made considering symptoms after the allergen injections.
All inclusion and exclusion criteria were checked at visits 1–5.
PEF: peak expiratory flow, SPT: skin prick test, U-HCG: urine human chorionic gonadotropin (only in females). ILIT: Intralymphatic immunotherapy.
FIGURE 1 Flow chart