Visit 1: Pre-ILIT, pre-season. Visit 2: pre-ILIT, post-season. Visit 3; 7-35d post visit 2. Visit 4: 28-42d post visit 3. Visit 5: 28-42d post visit 4. Visit 6: Fall year 1. Visit 7: Fall year 2. Visit 8: Fall year 3.

¹ Haematology: leukocytes, leukocyte differentiation (neutrophil, eosinophils, basophils, lymphocytes) haemoglobin and platelets at visits 1, 2, and 6. Coagulation blood tests at visits 1 and 2 (PK-INR and APTT).

² Total IgG and subsets (lgG1-G4), total IgE, allergen-specific IgE and IgG4, and other immunological tests.

³ RTSS, RQLQ and MS were measured after the birch pollen season (approx. Jun 1st) and after the grass pollen season (approx. Aug 1st)

⁴ The conjunctival challenge tests (CAPT) with timothy were performed according to the EAACI guidelines ²³. Due to lack of extract from the company planned CAPT were not performed after the third pollen season

⁵ The diary had space for description of the adverse events (AEs) since the last visit.

⁶ Two to five days after visit 3–5, telephone contact was made considering symptoms after the allergen injections.

All inclusion and exclusion criteria were checked at visits 1–5.

PEF: peak expiratory flow, SPT: skin prick test, U-HCG: urine human chorionic gonadotropin (only in females). ILIT: Intralymphatic immunotherapy.

FIGURE 1 Flow chart