FIGURE 4 Proportion (%) of the T regulatory (Treg) cells in the CD3+CD4+ populations after intralymphatic immunotherapy with birch and/or 5-grass allergen. Blood samples were collected at three timepoints: screening, one year after, and three years after treatment had finished. The proportion of Treg cells, defined as CD4dimCD25hiFoxP3+cells, increased over time in all treatment groups (a-c). The same trend was observed in the activated Treg cell population, defined as CD3+CD4+CD45RA-FoxP3++(d-f). p* < 0.05; p** < 0.01; p*** < 0.001 from Wilcoxon signed rank tests. The lines indicate median and interquartile range (IQR, 25th and 75th percentile values).