3.3| Effects of carbonic anhydrase and
Na+/H+ exchanger inhibitors
Increased expression of genes involved in Na+ uptake
could be a possible cause of the death of H. tridens and M.
japonicus in 513.3 mmol/L NaCl solution. To examine this possibility,H. tridens and M. japonicas were reared in 513.3 mmol/L
NaCl solution supplemented with acetazolamide or amiloride, an inhibitor
of CA and NHE, respectively. Amiloride significantly increased the
survival rate of H. tridens and M. japonicus , and
acetazolamide increased the survival rate of M. japonicus reared
in 513.3 mmol/L NaCl solution (Fig. 6B–D). The survival rate was also
higher in H. tridens reared in 513.3 mmol/L NaCl solution
supplemented with acetazolamide compared to 513.3 mmol/L NaCl solution,
but the results were not statistically significant (Fig. 6A).