Not sure we need this paragraph: The use of PLFA profiling provides a broad-scale, yet quantitative approach to coarsely assessing microbial community diversity. It can be used to determine ratios of fungi to bacteria, as well as gram-positive to gram-negative bacteria based on specific phospholipid fatty acid signatures in a given sample. Recently, studies have compared the insights gained through the use of PLFAs and those from amplicon-based sequencing studies. These analyses suggest that PLFAs are as sensitive or even more sensitive than metabarcoding approaches in detecting changes in microbial community structure \cite{Orwin_2018,Ramsey_2006}. When taken together, the use of PLFAs and amplicon sequencing improve resolution for detecting quantitative differences in community composition over sequence-based approaches alone. However, differences in the fatty acid signature produced by a microbial guild as a result of their biomass and/or environmental conditions must also be considered (Duncan et al. 2016; Galazzo et al. 2020))