2.2 Necropsy and Histopathology
After death, three (22388PT20, 22390PT20 and 22391PT20) of the five
rabbits were necropsied according to routine procedures. Tissue samples
were collected for histopathology (eyelid, genitalia, ear, lung and
kidney) and virology (liver, spleen, lung, eyelid and genitalia). The
other two rabbits were autolysed, therefore only submitted to
virological examination.
For histopathology, liver, spleen, kidney, eyelid, ear, muzzle and lung
fragments were fixated in 10% (w/v) neutral buffered formalin,
routinely paraffin embedded, sectioned at 4 µm, and stained with
Haematoxylin and Eosin (H&E).