Figure 2. IMC mapping identifies predominance of CD8+ T cell clusters in COVID-MDR.
A, UMAP representation of all single cells depicting the different identified cell-types (upper graph) and all cells colored by indication (lower graph). B, Boxplots for the fractions of each cell-type per image split and colored by indication. (COVID-MDR, MDR, DRESS, HC). C, Heatmap of z-scored average expression for each marker and cell-type. D, Example images of classified cell-types in COVID-MDR, DRESS and MDR. The scale bar (white line) is 200 µm in all images. E, Example images of the expression of CD3 (green), CD4 (magenta), CD8 (red), GrzB (yellow) and DNA (blue). The large images depict the overlayed colors, and the white box marks the zoomed in areas on the right side depicting the individual markers. The scale bar (white line) is 100 µm in all images.