Chloroplast DNA extraction
Mutational rice (B810S) with a color marker was obtained from a deserted
paddy field in Huaihua, Hunan Province, China. After collection, all
leaves from B810S and 810S were washed three times with physiological
saline, and were then initially identified by their morphological
characteristics using a light microscope. The genomic DNA contents of
B810S and 810S were isolated from their leaves using a Plant DNA
isolation Reagent kit (Tiangen, Beijing, China) following the manual
instructions, and were then stored at -20°C until use.