Chloroplast DNA extraction
Mutational rice (B810S) with a color marker was obtained from a deserted paddy field in Huaihua, Hunan Province, China. After collection, all leaves from B810S and 810S were washed three times with physiological saline, and were then initially identified by their morphological characteristics using a light microscope. The genomic DNA contents of B810S and 810S were isolated from their leaves using a Plant DNA isolation Reagent kit (Tiangen, Beijing, China) following the manual instructions, and were then stored at -20°C until use.