Effect of LCZ on cell growth and the blood-milk barrier
E. coli caused a significantly higher LDH release compared with
other groups. No significant difference in LDH concentration was
observed in the LCZ + E. coli and LCZ groups compared with the
Control group
(Figure
1A).
The change in TEER in BMECs treated with E. coli was negative and
significantly lower than that of the other groups; specifically, the
change in TEER was negative in the LCZ + E. coli group but
significantly less negative compared with the change in TEER observed in
the E. coli group. In addition, the change of TEER in the LCZ
group was significantly higher than that of the Control group (Figure
1B).
The desmosome structure in the E. coli treatment group was
severely damaged and blurred, as illustrated by TEM. Meanwhile, the
degree of damage to the desmosome structure alleviated was noticeably
greater in the LCZ + E. coli group compared with the E.
coli group. Moreover, the desmosome structure in the LCZ group was
clearly visible under TEM, and no clear difference was detected compared
with the Control group (Figure 1C).