Material
MRS broth, TSB, TSA, MRS agar, 4% cell tissue fixative and Beyo
Enhanced Chemiluminescence Reagent Kit were all purchased from Solarbio
Life Science (Beijing, China). DMEM-F12 medium, FBS, 1% non-essential
amino acids, 1% insulin-transferrin-selenium, PBS, BSA, and mouse
TNF-α, IL-6, and IL-1β ELISA kits were all purchased from ThermoFisher
Scientific (Waltham, MA, USA). 1% penicillin and streptomycin, Total
Protein Extraction Kit, and BCA Protein Assay Kit were purchased from
Beyotime Biotechnology (Shanghai, China). Bovine TNF-α, IL-6, and IL-1β
ELISA kits was purchased from DG Biotech (Beijing, China), 15 ng/mL EGF
was purchased from PeproTech (Cranbury, NJ, USA), and TBST was purchased
from Nobleryder (Beijing, China). Primary antibodies for western blot
(ZO-1, claudin-1, claudin-4, and occludin for BMECs) were purchased from
Bioss Antibodies (Beijing, China), and ZO-1, claudin-3, and occludin for
mice mammary tissue were purchased from Abcam, (Cambridge, UK). All
secondary antibodies for western blot were purchased from CoWin
Biosciences (Beijing, China).