TMUV genetic diversity and epidemiological data in China
To investigate the genetic diversity of TMUV, we used three different methods (NJ, ML, and MCC) to construct the phylogenies using the ORF sequence of the TMUVs. All of the strains represent four different avian species that were isolated in China from 2010 to 2019, and the strain XZW10694 (Accession number: KY937189) as outgroup. Three different clades were observed using the three different methods (Fig.2a, 2b, and 2c). Compared with a previous study (Dai, Li, & Tao, 2015), a new clade was observed in the three different phylogenetic trees. This new clade (clade 3) was isolated in Shandong in 2014 and the host was Anas platyrhynchos (Accession number: MH748542). Clade 3 showed a longer evolutionary distance compared to clades 1 and 2, which might suggest that clade 3 is a potential new genotype. Based on the BSP, the TMUV might have diversified from 2010 to 2012, after which it entered the platform period (Fig. 3).