Apocynin prevents the suppression of myogenic factor expression and myofiber wasting induced by CSE and H2O2
Under unstimulated conditions, apocynin treatment had no effect on myotube diameters (Figure 5A and F). However, treatment with apocynin prevented myofiber wasting (i.e. reduction in myofiber size) elicited by different concentrations of H2O2 (Figure 5A) or CSE (Figure 5F). Apocynin treatment completely attenuated the up-regulated expression of Nox2 (Figure 5B and G) and Il-6(Figure 5C and H) driven by H2O2 or CSE. Moreover, apocynin normalized the expression of Igf1-ea and Igf1-eb , suggesting apocynin was able to antagonize the oxidant-dependent and -independent effects of CSE on myofiber wasting.