Culture of M1 and M2 macrophages
The detail methods were as same as the process of cultured M2 macrophage
but M1 macrophage were added with LPS(10ng/ml, Solarbio) instead of
IL-4. BAY-11-7082(1 µM) were added on the day 8 of cultures and control
group were added with PBS, the wells were incubated for 48h, 20µl
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)
solution was added to each well and incubated for 4h. Then, 150µl
dimethylsulfoxide was added to each well and low-speed oscillation was
performed on the shaker for 10min to fully dissolve the crystals. The
absorbance value of each well was measured at 570nm by an enzyme-linked
immunodetection.