Figure 7. TEC treatment protects against ANIT-induced intrahepatic cholestasis dependent on activating PPARγ. C57BL/6J mice were treated as previously mentioned (Figure 1). (A) The serum levels of aspartate transaminase (AST), alanine transaminase (ALT) and AP were determined by an ELISA kit, n=6. (B) Representative sections stained with HE were used to estimate the area of necrosis. (C) Bile acid pool size was determined by ELISA kits in the liver, serum and feces, n=6. (D-F) The mRNA level of macrophage recruitment (F4/80), inflammatory factors (TNF-α, IL-1β, MCP-1 and CCR2) and fibrosis-related genes (TGFβ1, MMP9, TIMP-1 and col1α1) were measured by qRT-PCR, n=6. (G) qRT-PCR analysis was used to determine the mRNA level of Oatp, Ntcp, Mrp2, Bsep, ABCG5 and ABCG8, n=6. (H) Western blot analysis was used to confirm the expression of PPARγ, FXR and LXR, n=5. *p<0.05. The data represent the mean ± SD.