Marker |
Clone |
Isotype |
Conjugate |
Source |
Dilution |
CD2 |
MSA4 |
mouse IgG2a |
— |
in-house |
1:100 |
CD3ε |
PPT3 |
mouse IgG1 |
APC |
Southern Biotech |
1:500 |
CD3ε |
PPT3 |
mouse IgG1 |
PE |
Southern Biotech |
1:500 |
CD4a |
74-12-4 |
mouse IgG2b |
PerCp-Cy5.5 |
BD |
1 :100 |
CD8a |
76-2-11 |
mouse IgG2a |
FITC |
Southern Biotech |
1:100 |
CD8b |
PG164A |
mouse IgG2a |
— |
in-house |
1:1000 |
Foxp3 |
FJK-16s |
mouse IgG1 |
PE-Cy7 |
ebioscience |
1:200 |
gdTCR |
PPT16 |
mouse IgG2b |
— |
in-house |
1:100 |
Ki-67 |
B56 |
mouse IgG1 |
BV421 |
BD Biosciences |
1:40 |
Perforin |
dG9 |
mouse IgG2b |
Alexa 647 |
Biolegend |
1:20 |
T-bet |
4B10 |
mouse IgG1 |
APC |
Biolegend |
1:500 |
IgG2a |
polyclonal |
Goat IgG |
APC-Cy7 |
Southern Biotech |
1:250 |
IgG2b |
polyclonal |
Goat IgG |
PE |
Southern Biotech |
1:400 |
IgG2b |
polyclonal |
Goat IgG |
PE-Cy7 |
Southern Biotech |
1:400 |