Figure legends

Figure 1 T-cell subset variations in various tissues upon moderately virulent ASFV infection. At the indicated time points, domestic pigs (orange) and wild boar (blue) were euthanized and lymphocytes of the respective tissues were isolated. CD3+ lymphocytes were subdivided into γδ T cell receptor (TCR) αβ T cells and γδ TCR+ γδ T cells. Frequency of (A ) αβ and (B ) γδ T cells in blood, spleen, lung, liver, and ghLN. Each point represents data from a single pig while bars represent the means for the designated time points. Data from trial 1 (●), data from trial 2 (■). *, p < 0.05. ghLN, gastro-hepatic lymph node
Figure 2 Increasing frequencies of CD8α+ αβ T cells. At the indicated time points, domestic pigs (orange) and wild boar (blue) were euthanized and lymphocytes of the respective tissues were isolated. CD3+ αβ T cells were analyzed for CD4 and CD8α expression. Frequency of (A ) CD4/CD8α+ αβ T cells and (B ) CD4+/CD8α+ αβ T cells in blood, spleen, lung, liver, and ghLN. Each point represents data from a single pig while bars represent the means for the designated time points. Data from trial 1 (●), data from trial 2 (■). *, p < 0.05. ghLN, gastro-hepatic lymph node
Figure 3 Increasing frequencies of CD8α+ effector γδ T cells in wild boar. At the indicated time points, domestic pigs (orange) and wild boar (blue) were euthanized and lymphocytes of the respective tissues were isolated. CD3+ γδ T cells were analyzed for CD8α expression. Frequency of CD8α+ γδ T cells (A ) spleen, (B ) lung, (C ) liver, and (D ) ghLN. Each point represents data from a single pig while bars represent the means for the designated time points. Data from trial 1 (●), data from trial 2 (■). *, p < 0.05. ghLN, gastro-hepatic lymph node
Figure 4 Increasing frequencies of proliferating CD8αα+ and CD8αβ+ αβ T cells. At the indicated time points, domestic pigs (orange) and wild boar (blue) were euthanized and lymphocytes of the respective tissues were isolated. CD8α+ αβ T cells were subdivided based on their expression of the CD8β chain. Frequency of (A ) CD8αα+ and (B ) CD8αβ+ T cells in blood, spleen, lung, and liver. Each point represents data from a single pig while bars represent the means for the designated time points. *, p < 0.05.
Figure 5 Loss of perforin expression in cytotoxic T cells. At the indicated time points, domestic pigs (orange) and wild boar (blue) were euthanized and lymphocytes were isolated. (A ) CD8αα+ and CD8αβ+ αβ T cells, and (B ) CD2+/CD8α+ effector γδ T cells were investigated for perforin expression in the indicated tissues. Each point represents data from a single pig while bars represent the means for the designated time points. Data from trial 1 (●), data from trial 2 (■). *, p < 0.05. ghLN, gastro-hepatic lymph node
Figure 6 T-bet-dependent T-cell activation in wild boar. At the indicated time points, wild boar were euthanized and lymphocytes were isolated. (A ) CD4+/CD8α, CD4/CD8α+, and CD4+/CD8α+ αβ T cells (from left to right) as well as (B ) activated and effector γδ T cells were analyzed for their expression of T-bet. T-bet expression in domestic pigs for comparison can be found in Supplemental figure 2. Each point represents data from a single pig while bars represent the means for the designated time points. *, p < 0.05. ghLN, gastro-hepatic lymph node
Figure 7 FoxP3+ regulatory T cells appeared in domestic pigs and wild boar. At the indicated time points, domestic pigs (orange) and wild boar (blue) were euthanized and lymphocytes of the respective tissues were isolated. CD4+/CD8α or CD4+/CD8α+ αβ T cells were analyzed for their expression of FoxP3. Frequency of (A ) FoxP3+/CD4+/CD8αand (B ) FoxP3+/CD4+/CD8α+T cells in blood, spleen, and ghLN. Each point represents data from a single pig while bars represent the means for the designated time points. *, p < 0.05. ghLN, gastro-hepatic lymph node
Figure 8 Activation and differentiation of iNKT cells in domestic pigs. At the indicated time points, domestic pigs were euthanized and lymphocytes of the respective tissues were isolated. CD3+ mCD1d tetramer+ iNKT cells were analyzed by flow cytometry. Frequency of iNKT cells positive for (A ) ICOS, (B ) MHC II, (C ) CD8α, and (D ) CD4/CD8α. Each point represents data from a single pig while bars represent the means for the designated time points. *, p < 0.05. ghLN, gastro-hepatic lymph node
Supplemental figure 1 Proliferation of CD4+/CD8α and CD4+/CD8α+ αβ T cells in the spleen. At the indicated time points, domestic pigs (orange) and wild boar (blue) were euthanized and lymphocytes of the respective tissues were isolated. Frequency of (A ) CD4+/CD8α and (B ) CD4+/CD8α+ T cells in spleen. Each point represents data from a single pig while bars represent the means for the designated time points. *, p < 0.05.
Supplemental figure 2 No evidence of T-bet-dependent activation in domestic pigs. At the indicated time points, domestic pigs (orange) were euthanized and lymphocytes of the respective tissues were isolated. Frequency of T-bet+ cells in (A ) blood, (B ) spleen, (C ) liver, and (D ) ghLN. Each point represents data from a single pig while bars represent the means for the designated time points. *, p < 0.05. ghLN, gastro-hepatic lymph node
Supplemental figure 3 Frequency and CD25 expression of iNKT cells in domestic pigs. At the indicated time points, domestic pigs were euthanized and lymphocytes of the respective tissues were isolated. CD3+ mCD1d tetramer+ iNKT cells were analyzed by flow cytometry. Frequency of iNKT cells (A ) of CD3+ cells and (B ) positive for CD25. Each point represents data from a single pig while bars represent the means for the designated time points. *, p < 0.05. ghLN, gastro-hepatic lymph node