3.4 OME animal model showed activated PI3K/Akt/mTOR signaling
Western Blot detection of PI3K/Akt/mTOR pathway related protein expression (Figure 8) showed that compared with CON group, OME group had significantly increased p-mTOR and p-PI3K protein expression, suggesting that under the stimulation of allergens, phosphorylation of PI3K and mTOR occurs locally in the mucosa of the middle ear, and the PI3K/Akt/mTOR pathway is activated, resulting in the imbalance of Treg/Th17 and the local immune response of the middle ear.