Figure 6 After successful modeling, CON and OME rats were sacrificed, spleen was collected, and Treg and Th17 cell frequencies in spleen were analyzed by FCM.A was the representative flow cytometry pattern of CD4, CD25 and FOXP3 staining of Treg cell frequencies in the spleen of CON and OME rats.B was the representative flow cytometry figure of CD3, CD4 and IL-17 staining of Th17 cells in CON and OME spleen.B was the typical flow cytometry figure of Th17 cells staining.C is the histogram of proportion of Treg cells to CD4+ in spleen of rats in the two groups.D is the histogram of the proportion of Th17 cells to CD4+ in spleen of rats in the two groups.E is a histogram of the ratio of Treg to Th17 cells in the spleens of the two groups of rats.(*P<0.05, **P<0.01, ***P<0.001, ****P< 0.0001).