3.1 Endoscopic and histological observation of the middle ear
Under endoscopy, the eardrum of CON group was transparent without
obvious effusion in the drum room. In OME group, the eardrum was orange,
turbidized and thickened, with obvious effusion in the drum chamber
(Figure 3). HE staining showed that the tympanum ciliated mucosal
epithelium in the control group was thin, scattered in goblet cells, and
there was no obvious inflammatory cell infiltration in the lamina
propria. The mucosal layer of the experimental group was thickened, with
local congestion, tissue edema, glands dilated, eosinophil aggregation
of plasma cells, increased goblet cells and hyperchromatic nuclei
(Figure 4). The above experiments demonstrate the success of OME
modeling.