2.5 Western blot
The OME and CON groups of auditory vesicle middle ear mucosal tissues
were homogenized on ice for protein extraction which was then quantified
using BCA kit (Thermo Fisher, Waltham, Massachusetts, US) and
standardized to 2 g/ uL protein density followed by western blot
analysis.The ECL chemiluminescence detection system was used to
visualize protein bands. The protein expression was standardized to
GAPDH.