2.5 Western blot
The OME and CON groups of auditory vesicle middle ear mucosal tissues were homogenized on ice for protein extraction which was then quantified using BCA kit (Thermo Fisher, Waltham, Massachusetts, US) and standardized to 2 g/ uL protein density followed by western blot analysis.The ECL chemiluminescence detection system was used to visualize protein bands. The protein expression was standardized to GAPDH.