3.3 | Diversity analysis of the rhizosphere microbial community
A total of 1,079,408 and 1,675,540 high-quality sequences were analyzed for bacteria and fungi, respectively. In bacteria, the high, medium and low carbon sequestration rhizosphere soils contained 1704, 1641, and 1672 OTUs, respectively. A total of 1221 OTU sequences were common among the three kinds of samples (Figure 1 A). In contrast, 808, 654 and 838 fungal OTUs were identified from the high, medium and low carbon sequestration rhizosphere soil samples, respectively. Only 254 soil fungal OTU sequences were common among the three sample types (Figure 1 B). B). The OTUs of fungi in each sample were far fewer than those of bacteria.
The Chao and Shannon index of bacteria and fungi are shown in Figure 1. C,D. For bacteria, the Chao index of the different samples fluctuated between 1476 and 1521, and samples with high carbon sequestration had slightly higher values that were not significantly different from those of the other samples. The Chao index for fungi was approximately 400. The difference in the Chao index values between fungi and bacteria was significant (Figure 1C). On the other hand, the Shannon index for bacteria and fungi from the different families showed only slight differences, and the differences among samples were not significant (Figure 1D). These results indicate that overall, combined with the other parameters used to evaluate the microbial community α-diversity shown in Table S3, there was little difference in α-diversity among the rhizosphere microorganisms in the various samples.