Cytokines
Cytokine concentrations in the serum were measured by ELISA as
previously described (Chang et al., 2015;
Jones, Ackermann, Wille, Hunter, & Scott,
2002). Purified anti-mouse capture and biotinylated detection of Abs to
IL-12p70, IFN-γ, IL-4 were conducted. All Abs were obtained from BD
Biosciences.