Figure legends
Figure 1 . Schematic and process diagram representing end-to-end
continuous process for mAb production with implementation of total
flow-through integrated polishing process
Figure 2 . Changes throughout mAb harvest, capture, low pH virus
inactivation and polishing processes for a) HCP (ng/mg-mAb), b) DNA
(pg/mg-mAb), c) mAb (mg/mL) and d) aggregate (%).
Figure 3 . Transmembrane pressure of mAb virus filtration
flow-through fraction and buffer wash implemented in the end-to-end
continuous process shown in Figure 1 for three batches with a) pressure
plotted against time (min) and b) pressure plotted against throughput
(L/m2).
Figure 4 . Setup of viral clearance test for flow-through two
column chromatography (upper) and 280 nm UV absorbance profile of
flow-through output for MVM-spiked mAb run (lower).
Figure 5 . Setup of inline spiking viral clearance test for
integrated polishing process with virus filter connected to flow-through
two columns chromatography (upper figure) and transmembrane pressure
profile of virus filter loaded with virus-spiked mAb flow-through from
the two column step (lower figure).