Figure legends
Figure 1 . Schematic and process diagram representing end-to-end continuous process for mAb production with implementation of total flow-through integrated polishing process
Figure 2 . Changes throughout mAb harvest, capture, low pH virus inactivation and polishing processes for a) HCP (ng/mg-mAb), b) DNA (pg/mg-mAb), c) mAb (mg/mL) and d) aggregate (%).
Figure 3 . Transmembrane pressure of mAb virus filtration flow-through fraction and buffer wash implemented in the end-to-end continuous process shown in Figure 1 for three batches with a) pressure plotted against time (min) and b) pressure plotted against throughput (L/m2).
Figure 4 . Setup of viral clearance test for flow-through two column chromatography (upper) and 280 nm UV absorbance profile of flow-through output for MVM-spiked mAb run (lower).
Figure 5 . Setup of inline spiking viral clearance test for integrated polishing process with virus filter connected to flow-through two columns chromatography (upper figure) and transmembrane pressure profile of virus filter loaded with virus-spiked mAb flow-through from the two column step (lower figure).