RESULTS
In the present study, the mean age of patients was 24.23 ± 3.39 years,
(range 18.0 – 34.0years). The gestational age recorded by the
ultrasound at the time of blood sampling ranged from 8.14 – 13.71 weeks
with a mean of 10.91 ± 1.59 weeks. All the laboratory test results are
illustrated in Figure 1.
Figure 2 demonstrates the assessed prevalence of thrombophilia
polymorphisms in the studied cases: according to MTHFR C 677T mutations
only, the prevalence was in 17 cases (42.5 %), while FVL mutation only
prevalence was in 8 cases only (20.0 %). It was observed that 10% (4
cases) were combined MTHFR C 677T and FVL mutation, while, 27.5%. (11
cases) were normal.
The number of cases without MTHFR C 677T gene mutations was 19 cases
(47.5%). While the number of cases with MTHFR C 677T mutations was 21
cases (52.5%). It was observed that 15 cases out of the 21 cases (37.5
%) with MTHFR C 677T mutations were heterozygous. While 6 cases only
out of the 21 cases (15%) with MTHFR C 677T mutations were homozygous.
The number of cases without FVL gene mutations was 28 cases (70.0 %).
While the number of cases with FVL mutations was 12 cases (30.0%). It
was observed that all cases with FVL mutations were heterozygous (37.5
%), and there were no homozygous cases between the studied cases.
Regarding the distribution of the studied cases according
to MTHFR gene mutation and gestational age by the US (Table 1): There
was no case with MTHFR gene mutation between 7-8 weeks of gestations.
While there were 4 cases with MTHFR gene mutation between 8-9 weeks of
gestations (10 %), 3 cases were heterozygous and 1 case was homozygous.
There were 5 cases with MTHFR gene mutation between 9-10 weeks of
gestations (12.5 %), 3 cases were heterozygous and 2 cases were
homozygous. While there were 2 cases with MTHFR gene mutation between
10-11 weeks of gestations (5 %) and both were heterozygous. There was
there were 6 cases with MTHFR gene mutation between 11-12 weeks of
gestations (15 %), 4 cases were heterozygous and 2 cases were
homozygous. While there were 2 cases with MTHFR gene mutation between
12-13 weeks of gestations (5 %) and both were heterozygous. Finally,
there were 2 cases with MTHFR gene mutation between 13-14 weeks of
gestations (5 %) and 1 case was heterozygous and the other was
homozygous.
Regarding the distribution of the studied cases according
to FVL gene mutation and gestational age by the US
(Table 2): There was no case with homozygous FVL mutation in the studied
cases. There was no case with FVL gene mutation in the studied cases
between 7-8 weeks of gestations. While there was one case with FVL gene
mutation between 8-9 weeks of gestations, and one case between 9-10
weeks of gestations (2.5 %) for both. There was there were two cases
with FVL gene mutation between 10-11 weeks of gestations (5 %), and 3
cases were between 11-12 weeks of gestations (7.5 %). While there were
2 cases with FVL gene mutation between 12-13 weeks of gestations (5 %),
and 3 cases were between 13-14 weeks of gestations (7.5 %).
Comparison between MTHFR and FVL showed that there was
about 19 case with no gene mutation related to MTHFR while about 28
cases with normal FVL so cases with normal study related to FVL more
than case related to MTHFR. There were about 15 heterozygous cases of
MTHFR gene mutation which more than heterozygous cases with FVL which
was about 12 cases. There was no case with homozygous mutation related
to FVL while was there about 6 cases with homozygous related to MTHFR
gene mutation. There was a significant statistical difference between
MTHFR and FVL with p vale = 0.022*.
The relation between MTHFR and FVL revealed that there
were about 11 cases with no MTHFR or FVL gene mutation. There were 11
cases of heterozygous gene mutation of MTHFR while normal FVL. There
were 6 cases with homozygous gene mutation of MTHFR while normal FVL.
There were 8 cases with heterozygous gene mutation of FVL while normal
MTHFR gene. There were 4 cases with heterozygous gene mutations of both
the MTHFR gene and FVL gene. There was no significant statistical
difference between MTHFR and FVL with p vale = 00.168