Statistical model
Statistical analysis and the relative graphs were made using GraphPadPrism 6 (GraphPad Software Inc., La Jolla, CA, USA).
The statistical significance of the cytofluorimetric analyses were assessed used using Student’s t-test, the number of single cell events analyzed was never less than 9,980 cells out of 10,000 events at the outset. The significance threshold was set p -value ≤0.05 (significant), p -value ≤0.01 (highly significant), andp -value ≤0.001 (extremely significant) (Bongiorno, Musso et al., 2020). The evaluation of the MTT assay statistical significance was assessed using ANOVA and Sidak’s multiple comparisons test (post hoc test). The expression analysis statistical significance was assessed using Ordinary one-way ANOVA and Bonferroni’s Multiple Comparison Test.