Figure legend:
Figure 1 . Evaluation of the internalization frequency by IFC
evaluation and cellular metabolism viability, after 3h p.i. at an MOI of
100:1. A. Percentage of spots per cell ± SD for each strain for
three different experiments. B . Detailed statistical data
obtained using Student’s t-test vs ATCC12598: p-value, 95% confidence
and R squared. Statistically significant p-value ≤0.05 *; highly
significant ≤0.01**; extremely significant ≤0.001***.
Figure 2 . Evaluation of the intracellular persistence by IFC
evaluation and cellular metabolism viability, after 24h p.i. at an MOI
of 100:1. A. Percentage of spots per cell ± SD for each strain
for three different experiments. B. Detailed statistical data
obtained using Student’s t-test vs ATCC12598: p- value,
95% confidence and R squared. Statistically significant p -value
≤0.05 *; highly significant ≤0.01**; extremely significant ≤0.001***,
more than extremely significant ≤0.0001****.
Figure 3 . Evaluation of the relative mRNA expression of genes
involved in regulation in ATCC12598, ST239 and ST228 strains after 3h
and 24h p.i. vs basal condition. Horizontal bars report
statistical analysis conducted vs ATCC12598 and between ST239 and ST228.
Statistically significant p -value ≤0.05 *; highly significant
≤0.01**; extremely significant ≤0.001***.
Figure 4 . Evaluation of the relative mRNA expression of the
MSCRAMM genes in ATCC12598, ST239 and ST228 strains after 3h and 24h
p.i. vs basal condition. Horizontal bars show statistical
analysis conducted vs ATCC12598 and between ST239 and ST228.
Statistically significant p -value ≤0.05 *; highly significant
≤0.01**; extremely significant ≤0.001***.
Figure 5 . Evaluation of the relative mRNA expression of toxin
genes in ATCC12598, ST239 and ST228 strains after 3h and 24h p.i.vs basal condition. Horizontal bars show statistical analysis
conducted vs ATCC12598 and between ST239 and ST228. Statistically
significant p -value ≤0.05 *; highly significant ≤0.01**;
extremely significant ≤0.001***.