Intranasal OT attenuated the DSS-induced changes in macrophage
cell, NK cell and Treg cell populations in the spleen
To investigate the mechanism underlying the effects of intranasal OT on
IBD mice, we performed a flow cytometry analysis of M1 and M2 type
macrophages, NKp46+NK cells and Treg cells isolated from spleens
obtained from mice in the different treatment groups. DSS treatment
remarkably augmented the percentages of the M1 and M2 type macrophages
in the spleen, while it simultaneously decreased the percentage of Treg
cell (Fig.5A, B and D). Intranasal OT administration further increased
the percentages of M1 and M2 type macrophages in the spleen, increased
the percentage of Treg cells and decreased the M1/M2 ratio and the
percentage of NKp46+NK cell (Fig.5A-D).