Statistical Analysis
The visualized patterns of western blot were analyzed by ImageJ 1.48 and Image Lab 4.1.0 to transfer images to digits. All data, each one representing the outcome of a single animal, were presented as means ± SEM. Comparisons among experimental groups were made using analysis of variance (one-way or two-way ANOVA) followed by the Tukey-Kramer multiple comparisons post-test when P  < 0.05, chosen as level of statistical significance, was achieved. All analyses were performed using Prism 4 software (GraphPad Software Inc., San Diego, CA, United States).
Result