Water avoidance stress test (WAST)
We performed water avoidance stress test as previously described23.The equipment of water avoidance stress was used to provide an assessment of stress response. After 7-day drug treatment, each group of mice in behavioral test were placed on a small platform in the center of water-filled tank, the level of the water (25 °C) in the tank being 1 cm below the platform. The stress procedure was carried out in a brightly lit room. Immediately after submission to WAS, the behavior of mice was recorded for 10 min to evaluate their behavioral reaction to stress and recorded. Time being moving, time being immobile and time spent on grooming on the platform were measured. After exposure to WAST, the mice were returned to their cage.