Splenectomy
Splenectomy was performed 15 days
before DSS-induced colitis. Mice were fasted for eight hours before
surgery. Then mice were anesthetized by intraperitoneal injection of
pentobarbital 50 mg/kg. The spleen was removed after abdominal surgery
and ligation of blood vessels. After the surgical intervention, mice
were monitored daily to examine their health state. We reconfirmed the
absence of spleen when the specimens were retained.