ELISA
BDNF (CUSABIO, #CSB-E04505m), Cortisol (CUSABIO, # CSB-E05113m), ACTH (CUSABIO, #CSB-E06874m), CRH (CUSABIO, #CSB-E14068m), catecholamine (Fitzgerald, #3-CAT ELISA Kit), OT (CUSABIO, #CSB-E09245m), Ach(CUSABIO, #CSB-E04744m) ,TNF-α(CUSABIO, #CSB-E04744m) and IL-1β(CUSABIO, CSB-E08054m)was detected by using relevant ELISA kit following the manufacturer’s instructions.