Results
High-throughput sequencing of 16S rRNA and ITS2 amplicon libraries
produced a total of 734,157 and 677,115 high-quality reads,
respectively. Clustering of similar sequences (>97% of
identity) resulted in 1,241 and 933 OTUs, respectively. Alfa and beta
diversity metrics highlighted significant differences of conjunctival
bacterial and fungal microbiota composition in VKC patients and HC.Proteobacteria , Firmicutes and Actinobacteria phyla
were present in all subjects qualifying theme as a putative core
microbiome of both HC and VKC groups. In addition, Bacteroidetesand Fusobacteria met the core microbiome’s definition criteria in
VKC patients. Of the 132 observed families, Moraxellaceae showed
a higher abundance in VKC group than HC. Saccharomycetaceae,
Malasseziaceae , and Dipodascaceae were present in all subjects,
constituting the fungal core microbiome of both HC and VKC patients.
OTUs referred to Malasseziaceae were significantly higher in VKC
children compared to HC.