atrogularis.
Of the 77 sequences, 30 were non-translatable due to indels or the
presence of stop codons resulted changes in the reading frame. Sequences
were thus presumed to be pseudogenes. The number of identified
pseudogenes within the four species ranged between three to five in most
individuals of study population and six of the thirteen pseudogene
sequences were found to be identical in three individuals from the
population of sampled species. We cannot ignore the likelihood that some
of the identified pseudogene sequences may be due to PCR or sequencing
artifacts, as such events would more often result in nonfunctional
sequences. The nucleotide deletion result in loss of 3 amino acids was
obvious in Tuna-MHCI*PS 07-9 and Tueu-MHCI*PS 01-04 andTueu-MHCI*PS 08. Both nucleotide deletion, frame shift mutation
and premature stop codons were detected in Turu-MHCI*PS 01,03 andMHCI*PS 09 at amino acid 33 encoding Exon 3. Loss of 3 amino acids
was at position 78 was detected in Tuat-MHCI*PS 05 andTuat- MHCI*PS06.