Note: NS indicates not significant. Maj P and Min P represent as major
and minor parents. BP denote Breakpoint. The numbers in parentheses are
BP locations in the recombinant nucleotide sequences without gaps. The
values after the semicolon are Max Chi values for that BPs. **indicateP < 0.01.
Analysis of Selection
Considering that the evolutionary history of each domain might have been
different, we tested each domain separately for evidence of positive
selection. Selection statistics by traditional methods did not disclose
any statistical significant signal of selection that deviate from
neutral expectations for turdus enumus (Tajima’s D: -0.87309, P
> 0.10; Fu & Li’s D* test statistic: 0.36, P
> 0.10; Fu & Li’s F* test statistic: 0.03, P
> 0.10) and turdus atrogularis (Tajima’s D:
-0.86107, P > 0.10 Fu & Li’s D* test statistic: 0.19, P
> 0.10; Fu & Li’s F* test statistic: -0.077, P
> 0.10). Still, overall dN value was
significantly higher statistically than dS in Turdus
atrogularis ( 1.687) and ratio dN/ds was more
pronounced at codons presumably coding PBR (1.994) than codons not
involved in such activity (0.884) is presented in (Table 3).
TABLE 3: The average rates of nonsynonymous
(dN), synonymous (ds) substitutions and the result of
Z-test, the average nucleotide distances (dnt) and amino
acid distances (daa) for PBR, non-PBR and all sites in
MHC class I of Turdidae family.