FIGURE 5: The phylogenetic networks of MHC class I of four species of turdidae family along with homologues sequences from passerines species. GenBank accession number are provided. Species name are mention lower right side with branch colors. Neighbor-Net networks based on uncorrected P -distances and carried out 1000 bootstrap replicates to estimate nodal support. Nodal support values (> 75%) were displayed.
CONCLUSION
Our study shows that species of Turdidae family has retained significant MHC class I diversity, which supports high conservational value and contribute to evolution of MHC class I genes. Importantly, we specifically amplify the exon 3 locus, provide an opportunity to avoid chimera formation during molecular characterization of hypervariable genes of immunity. At the same time, our study is the first to validate contrasting patterns of allelic diversity and positive selection upon inferred PBR and non-PBR codons supported the hypothesis that different mechanisms can shape evolutionary paths of MHC class I.