FIGURE 2: Positively selected sites using online adoptive
evolutionary server Datamonkey with (a) MEME. (b) FEL.(c) FUBAR and (d) SALC. Substitution tests identify
numerous codons showed the signature of positive selection.
Across all tests for positive selection, four codons (9,29,65 and 88)
were frequently identified by all methods as having under positive
selection. Of these, codons (42, 59) were corresponding to PBR in human
and codons 9, 29, 64 and 88 also match homology to PBR, known as
positively selected among passerine in general [56] (Figure 4). The
ten most frequent MHC class I alleles retrieved from sampled species
displayed 87%-91% sequence similarity to 18 sequences from five other
passerine families(Acrocephalidae,
Passeridae,
Muscicapidae, Paridae, Passerellidae). None of the 77 alleles studied
had 100% sequence similarity to other published sequences to GenBank;
thus, it establishes no allelic pair in the study population that was
100 % sequence likeness shared by another species.