2.5.2 Inference of recombination:
Recombination can influence the outcomes of selection, we first tested recombination. Analyses were implemented for the nucleotide alignment of exon 3 in the Recombination Detection Program version 4 (RDP4). Several method, including RDP [33], GENECONV [34], Chimaera [35], MaxChi [36], BootScan [33], SiScan [37], and 3Seq [38], were used to detect recombination events. In addition, the online GARD tool, provided by the Datamonkey webserver (http://www.datamonkey.org/), was used for recombination signals assessment [39].