2.2 Extraction of genomic DNA
Region of calamus to the rachis of contour feathers was excised, tissues
from skeletal muscles were minced, placed into a 1.5 ml Eppendorf tube
containing TNE buffer (10mM Tris-HCl (pH 8.0), 150mM NaCl, 2mM EDTA, 1%
SDS). Total genomic DNA was extracted with AxyPrep Multisource Genomic
DNA Miniprep Kit (AXYGEN, China) according to the manufacturer’s
instructions. The DNA concentration was measured with Nanopore
Spectrophotometer at 260 nm absorbance. Samples above 100ng/µl
concentration were used for further analysis.