2.4 Definition of Allele
Since few artifacts introduced during the recombination of PCR products in cloning [25, 26]. Amplification, cloning and sequencing were performed twice. Sequences were verified and referred to as an Allele; either minimum of three sequences have the same nucleotide composition or repeated in both events. The sequences which showed any deletion, insertion, or premature stop codons within exons were identified as presumed pseudogene sequence, and others were considered as putative functional allele (PFA) [27]. All sequences appropriate to our criteria have been deposited into the GenBank (Accession No: MN849308-54).