FIGURE 2: Positively selected sites using online adoptive evolutionary server Datamonkey with (a) MEME. (b) FEL.(c) FUBAR and (d) SALC. Substitution tests identify numerous codons showed the signature of positive selection.
Across all tests for positive selection, four codons (9,29,65 and 88) were frequently identified by all methods as having under positive selection. Of these, codons (42, 59) were corresponding to PBR in human and codons 9, 29, 64 and 88 also match homology to PBR, known as positively selected among passerine in general [56] (Figure 4). The ten most frequent MHC class I alleles retrieved from sampled species displayed 87%-91% sequence similarity to 18 sequences from five other passerine families(Acrocephalidae, Passeridae, Muscicapidae, Paridae, Passerellidae). None of the 77 alleles studied had 100% sequence similarity to other published sequences to GenBank; thus, it establishes no allelic pair in the study population that was 100 % sequence likeness shared by another species.