2.4 Definition of Allele
Since few artifacts introduced during the recombination of PCR products
in cloning [25, 26]. Amplification, cloning and sequencing were
performed twice. Sequences were verified and referred to as an Allele;
either minimum of three sequences have the same nucleotide composition
or repeated in both events. The sequences which showed any
deletion, insertion, or premature
stop codons within exons were identified
as
presumed pseudogene sequence, and others were
considered
as
putative
functional allele (PFA) [27]. All sequences appropriate to our
criteria have been deposited into the GenBank (Accession No:
MN849308-54).