Plant growth and tissue preparation
A. thaliana Col-0, fum2.2 , and C24 genotypes were grown in
a multi-purpose peat-based compost in 3-inch pots with 8-hour
photoperiods at 20 °C and under a 100 μmol m-2s-1 irradiance, as previously described (Dysonet al ., 2016). Light was provided by warm white LEDs (colour
temperature 2800-3200 K). Adult plants (8-9 weeks) were then either kept
at 20 °C for up to another week (control treatment) or transferred to 5,
10, 15, 25, or 30 °C, under identical irradiance and photoperiod
conditions. Fully expanded leaves were collected at the beginning and at
the end of the photoperiod on the 1st and
7th day of the different treatments, flash-frozen in
liquid nitrogen, freeze-dried and stored at -20 oC for
metabolite assays. Leaves were also collected without freezing and their
fresh and dry weights per unit area recorded.