Plant growth and tissue preparation
A. thaliana Col-0, fum2.2 , and C24 genotypes were grown in a multi-purpose peat-based compost in 3-inch pots with 8-hour photoperiods at 20 °C and under a 100 μmol m-2s-1 irradiance, as previously described (Dysonet al ., 2016). Light was provided by warm white LEDs (colour temperature 2800-3200 K). Adult plants (8-9 weeks) were then either kept at 20 °C for up to another week (control treatment) or transferred to 5, 10, 15, 25, or 30 °C, under identical irradiance and photoperiod conditions. Fully expanded leaves were collected at the beginning and at the end of the photoperiod on the 1st and 7th day of the different treatments, flash-frozen in liquid nitrogen, freeze-dried and stored at -20 oC for metabolite assays. Leaves were also collected without freezing and their fresh and dry weights per unit area recorded.