2.2. Detection of Common Enteric Pathogens by Enzymatic Immunoassay (EIA)
To identify the possible etiological agent of calf diarrhea, 18 specimens from ill calves collected during the peak of the diarrhea outbreak were examined for four common enteric pathogens. For this, 200 mg frozen fecal material was tested for rotavirus, coronavirus,Escherichia coli , and Cryptosporidium spp. using the Pathasure Enteritis EIA kit (Biovet, Saint-Hyacinthe, Canada). In addition, rotavirus in another 75 fecal specimens collected during (n = 37) and after (n = 38) the diarrhea outbreak from 1 to 4-week-old calves were tested using the ProSpecT Rotavirus Kit (Thermo-Oxoid, Basingstoke, UK).