Acknowledgments
We acknowledge GISAID for sharing the sequence data and IDT for giving the opportunity to use the tools for validating primers in silico . We also acknowledge Ministry of Health and Family Welfare, Bangladesh for giving us the permission for SARS CoV-2 diagnosis, as well as, the COVID-19 diagnostic team of Jashore University of Science and Technology, Jashore-7408, Bangladesh for their contribution to initial experiments on raw samples.