Validation of multiplex PCR assays
All the 24 positive samples confirmed the test reproducibility of the
assays; four of them excluding the one used before for multiplex assays
were taken as representative to display the reproducibility in this
article (Fig.2 IIIA-IIIC ). In this study, only GR clade was
found in all positive samples tested. The homology of the nucleotide
sequences for the PCR products showed more than 99% identity with the
respective positions of the clades that validated the assays
(supplementary Fig.s2 ). Accession IDs to the submitted
sequences for one positive sample as an archetype are available in
GISAID EpiFlu™ database (EPI_ISL_548260, EPI_ISL_561630,
EPI_ISL_561375, EPI_ISL_561376 and EPI_ISL_561377).