Statistical analysis
Frequencies of lymphocyte subsets and usage of each TCR-Vβ family between patients with AIN and age-matched control subjects were compared using the Wilcoxon rank-sum test. In the analysis of the skew of usage of TCR-Vβ families, we used the χ2 test and Wilcoxon rank-sum test. The Wilcoxon signed-rank test was used to analyze the different usages of TCR-Vβ families between conventional T cells and Tregs. Two-sided p-values < 0.05 denoted statistically significant differences. Statistical analysis was performed using the JMP12 (SAS Institute Inc., Cary, NC, USA) software.