2.5 Genetic diversity and structure analysis
A total of 51 loci were obtained. The linkage disequilibrium of each locus was detected using GENEPOP software (Raymond & Rousset, 1995), after which the loci that presented linkage disequilibrium were removed. Nei’s gene diversity index (H), Shannon phenotype information index (I), population differentiation coefficient (G ST), and the percentage of polymorphic bands (PPL ) were calculated using the POPGENE software package (Francis et al., 1999).
The degree of molecular variation and the distribution pattern of genetic variation were analyzed using ARLEQUIN software (Expoffer & Lischer, 2010).