2.5 Genetic diversity and structure analysis
A total of 51 loci were obtained. The linkage disequilibrium of each
locus was detected using GENEPOP software (Raymond & Rousset, 1995),
after which the loci that presented linkage disequilibrium were removed.
Nei’s gene diversity index (H), Shannon phenotype information index (I),
population differentiation coefficient (G ST), and
the percentage of polymorphic bands (PPL ) were calculated using
the POPGENE software package (Francis et al., 1999).
The degree of molecular variation and the distribution pattern of
genetic variation were analyzed using ARLEQUIN software (Expoffer &
Lischer, 2010).