Serum biochemistry
Blood was collected from C57BL/6 mice in tubes with heparin after cardiac puncture. The levels of alanine aminotransferase (ALT), aspartate transaminase (AST), lactate dehydrogenase (LDH), and creatinine were quantified by spectrophotometric analyzer (modular DP; Roche-Hitachi, Echevarne Laboratories).