Measurement of cytokines and chemokines
The concentrations of CXCL16 and KC (IL-8 murine homolog) were quantified using commercially available enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems). The levels of other inflammatory cytokines and chemokines including IL-6, IL-8, IL-27, and TNF-α were assessed with commercially available ELISA kits (Biolegend). In order to quantify cytokines and chemokines in peritoneal lavage fluid (PLF), 5 ml of cold PBS was injected into the peritoneal cavity. PLF was then collected for further analysis.