Measurement of cytokines and chemokines
The concentrations of CXCL16 and KC (IL-8 murine homolog) were
quantified using commercially available enzyme-linked immunosorbent
assay (ELISA)
kits
(R&D Systems). The levels of other inflammatory cytokines and
chemokines including IL-6, IL-8, IL-27, and TNF-α were assessed with
commercially available ELISA kits (Biolegend). In order to quantify
cytokines and chemokines in peritoneal lavage fluid (PLF), 5 ml of cold
PBS was injected into the peritoneal cavity. PLF was then collected for
further analysis.