Fluorescence microscopy
For confocal microscopy, control or SQR9 VCs-treated transgenicArabidopsis seedlings (pPIN1:PIN1:GFP ,pPIN2:PIN2:GFP , pPIN3:PIN3:GFP and pPIN7:PIN7:GFP )
were mounted in DDW on microscope slides. A Leica SP8 laserāscanning
microscope was used for fluorescence imaging of the Arabidopsisroots. Chromophores were excited using a 488-nm argon laser, and
fluorescence was detected at 500 to 550 nm. More than eight independent
seedlings were analyzed per line, and treatment representative images
were selected for figure construction.