Product Quality Analysis
The harvested cultures of fed-batch samples were purified by either one-step protein A chromatography capture (shake flask samples) or WuXi Biologics downstream platform (manufacturing samples). Purified samples were subjected to WuXi Biologics platform analytical methods, such as Size Exclusion Chromatography (SEC), imaged Capillary Isoelectric Focusing (iCIEF), and N-Glycan LC.