SARS-CoV-2 Neutralization Test
Neutralizing antibodies were evaluated by titration of sera against SARS-CoV-2 pseudotyped Vesicular Stomatitis Virus (VSV). The test is based on VSV-ΔG*FLuc pseudotyped with SARS-CoV-2-Spike-ΔER which correlates with SARS-CoV-2 neutralization as described previously.13,14 Pseudoviral titers were determined by limited dilution and fluorescence microscopy. For all samples, a fixed inoculum of 25,000 ffu was neutralized for 1 h and luciferase activity was determined 20 h post infection of HEK293T-ACE2+-cells. IC50 values were fitted using the algorithm: ‘log (inhibitor) vs. normalized response’ and further analyzed in GraphPad Prism 8 software (GraphPad Software, San Diego, USA).