Omics
Ionomics and metabolomics were performed ICP-OES and by GC-MS, respectively, as in (Cui et al. , 2019a). Proteomics analysis was carried out as in (Cui et al. , 2019b) on total protein obtained by trichloroacetate-acetone extraction. Peptide identification and quantitation was done using the same workflow, except that protein abundance reported here are from extracted chromatograms (XIC). Details are provided in Supplementary methods S1.