Figure 2. Comparison of culture vessel for T cell products . Co-cultures of apheresis MNC and autologous LCLs were set up (n=6 donors) to compare culture in Corning flasks versus G-Rex100 flasks through an optimized three stimulation round protocol. Harvested samples were compared for phenotypic and functional profiles as before. (A ) Cultures from G-Rex flasks had a significantly higher percentage of CD8+ cells than standard flask grown lines (p<0.05). (B ) CD8+ cell phenotype was consistent between both culture methods for all markers analysed. Intracellular analysis of the gated CD8+/CD45RO+ cells showed no difference between standard flask and G-Rex cultured T cells for (C ) expression of cytokines by cMFI or (D ) relative frequencies of differentiated subpopulations by cytokine expression. Data are represented as means ± SEM.