Figure
Legends
Figure 1: Growth and metabolic characteristics of WT and BCAT1
KO clones in fed-batch cultures. A.) Viable Cell Density, B.)
Viability, C.) Lactate, D.) Glucose, E.) Titer, F.) Specific
Productivity, G.) isovalerate H.) isobutyrate, I.) 2-methylbutyrate.
BCAT1 KO Clone 83 (), BCAT KO Clone 90 ( ), WT Clone 47 ( ). For WT
Clone 47, solid and dotted lines represent duplicates.
Figure 2: Growth and metabolic characteristics of WT and BCAT1
KO clones in HiPDOG cultures. A.) Viable Cell Density, B.) Viability,
C.) Lactate, D.) Glucose, E.) Titer, F.) Specific Productivity, G.)
isovalerate H.) isobutyrate, I.) 2-methylbutyrate. BCAT1 KO Clone 83 (),
BCAT KO Clone 90 ( ), WT Clone 47 ( ). For WT Clone 47, solid and dotted
lines represent duplicates.
Figure 3: Levels of other metabolic byproducts across fed-batch
and HiPDOG cultures of BCAT1 KO clones. A.) 3-phenyllactate, B.)
4-hydroxyphenyllactate, C.) indole-3-lactate, D.) butyrate, E.) formate
and F.) glycerol concentrations in fed-batch and HiPDOG cultures of
BCAT1 KO Clone 83 () and BCAT1 KO Clone 90 ( ). Solid and dotted lines
represent fed-batch and HiPDOG cultures, respectively.
Figure 4: Effect of lactate on growth of CHO shake-flask batch
cultures. A.) BCAT1 KO Clone 83 and B.) WT Clone 47 viable cell density
over 6 days at added lactate concentrations ranging from 0 to 10 g/L.
Lactate concentrations gradually increased by roughly 2 g/L during the
culture for control, osmolality control and 2.5 g/L lactate conditions
but did not change substantially for higher concentrations (data not
shown). All conditions were run in triplicate. Error bars represent one
standard deviation.