Figure caption.
Figure 1 . Spatial distribution of sample collection. Each black
dot represents the province of sampling. The current geographic
distribution and census of beef cattle raised under extensive husbandry
conditions for each province is represented by grayscale. The number of
fecal samples per province is shown. The white dot indicates thatBesnoitia spp. DNA has been found.
Figure 2 . Phylogenetic relationships among Besnoitiaspp. sequences identified in this study and known Besnoitia spp.
isolates, as inferred by a neighbour-joining analysis of ITS ribosomal
RNA gene partial sequences, based on genetic distances calculated by the
Kimura two-parameter model. Nucleotide sequences determined in this
study are identified with dark green filled circles. Bootstrap values
lower than 50% are not displayed. Toxoplasma gondii was used as
outgroup taxa.
Table 1 . Summary of the available studies reporting
investigations to elucidate possible definitive host of Besnoitia
besnoiti .
Table 2 . Wild carnivore species examined, region, number of
samples tested and number of samples in which DNA from Besnoitiaspp. has been detected in Spain. In bold the species and number of
faeces samples in which DNA from Besnoitia spp. has been found
and confirmed by sequencing.