3.3. Mitochondrial lineage determination by mtDNA Cyt b PCR-RFLP
The mitochondrial lineage was identified in 81 male and female P. sergenti , 41 from El Borouj and 40 from Sidi Hajjaj. The results are shown in Table 3.
The female P. sergenti from El Borouj in which L. tropicaDNA was detected was identified as a lineage IV specimen. In addition, 4 positive females for L. tropica from captures made in 2014 (Gijón-Robles et al., 2018) were also identified as lineage IV.