2.3. Sand fly DNA extraction
Genomic DNA was extracted from the head, thorax and attached anterior
abdomen of individual P. sergenti males and females
(Martín-Sánchez et al., 2000). A commercially available kit was
used (RealPure kit from REAL (Ref. RBMEG01), according to the
manufacturer instructions. Each sandfly was individually placed in a
sterile 1.5 ml Eppendorf tube and kept in liquid nitrogen for a few
seconds to facilitate the mechanic rupture of the tissues using a
pestle. The DNA was resuspended in 20 µl of bidistilled water and kept
at -20 °C until use.