Figure S1. Expression of reference gene. We confirmed that our reference genes are expressed consistently across the treatments (ANOVA, Tub: Hours after mating, F 3,11 = 0.33, P = 0.806, Exp: F 1,11 = 0.98, P = 0.344, Hours after mating × Exp, F 3,11 = 0.39, P = 0.760, UbiE: Hours after mating, F 3,11 = 0.62,P = 0.618, Exp: F 1,11 = 0.47, P = 0.509, Hours after mating × Exp, F 3,11 = 0.22,P = 0.880).