5 CONCLUSION

The present study presented the first data investigating T. gondii genotyping from Gallus gallus domesticus, and revealed the existence of a large amount of atypical genotypes, in special in South America. Some studies show new genotypes never described before, which reinforces the idea that some years from now, even more new genotypes will be isolated, due to progressive genetic recombination.
The large amount of not defined genotypes due to inconclusive markers suggests the need of Nested-PCR technique in genotyping studies. The knowledge of the genotyping of the strains prevailing in free-range chickens denotes the virulence patterns found in each continent. The lack of data in Continents such as Europe, Asia and Oceania makes it necessary to perform new isolating and genotyping studies in these places.