This study was conducted to evaluate the antimicrobial activity of tow extract of Eryngium creticum plant belonging to the Apiaceae family collected from the coastal surrounding, Tartous, Syria. Many efforts have been made to discover new antimicrobial activity from a variety of sources such as microorganisms , animals and plants. One such source is Folk medicine. Systematic screening of them may result in the discovery of new effective compounds. These extracts were prepared with polar solvent (methanol and ethanol) by steeping extraction method. The antimicrobial activity of the extracts was assessed using the disk diffusion method against gram-positive (Staphylococcus aureus), and gram-negative (Escherichia coli) bacteria. The methanolic extracts of Eryngium creticum have shown antimicrobial activity against tested bacteria. The activity was compared with potent broad spectrum antibiotics such as Cefipime, Amecasine and ceftriaxon.