T1109, T1110
(section 2.1)
|
Wild type and interface mutant for
isocyanide hydratase
|
20-residue long domain swap induced by a single-point mutation
|
Environment difference: mutation
|
High:
1.0 & 0.7 Å resolution,
X-ray
|
Conformational change reproduced by groups from 7 labs
|
T1158, v0-4
(section 2.2)
|
Multidrug- resistant ABC transporter
|
Apo and complexed with each of four ligands, 3 distinct
conformations
|
Environment difference: ligands
|
Medium:
2.7 -3.5 Å, cryo-EM
|
Individual conformations reproduced by a few groups
|
T1160, T1161
(section 2.3)
|
Two 48-residue ‘ancient’ sequences, differing by five mutations
|
Crystallization condition and mutation-induced conformational
differences
|
Environment difference: mutations, crystal conditions
|
High:
1.3 Å,
X-ray
|
Individual conformations reproduced by one and three groups,
respectively
|
T1195-T1197
(section 2.4)
|
Three kinases
|
1 or 2 low occupancy alternative conformations each
|
Low population conformations
|
Low:
NMR CEST data, implied coordinates
|
Appropriate functional motifs sampled
|
R1156
(section 2.5)
|
135-base RNA
|
4 independent electron density maps, 10 map-derived models each
|
Experimental uncertainty: ensemble
|
Medium:
5.8 Å best resolution, cryo-EM
|
One model more accurate than experimental uncertainty
|
R1138
(section 2.6)
|
720-base designed RNA
|
Mature and folding intermediate
|
Folding intermediate
|
Medium:
4.8 & 5.2 Å,
cryo-EM
|
Intermediate not reproduced
|
T1189/R1189T1190/R1190
(section 2.7)
|
Protein/RNA complex
|
RNA with three (1189) or two (1190) protein dimers bound.
|
Two alternative oligomeric states
|
Medium:
3.8 & 4.6 Å, cryo-EM, two particle sets
|
Neither complex reproduced
|
T1170,
H1171 (v1,2), H1172 (v1-4)
(section 2.7)
|
Holliday junction complex in different states
|
Components include DNA, and/or organic ligands. Interdomain
differences
|
Time dependent differences: protein binding partners, ATP/ADP
|
Medium:
3 Å best resolution, cryo-EM
|
Not reproduced
|