Statistical analysis
Statistical analysis was performed using GraphPad Prism 8 Software (GraphPad Software, La Jolla, CA). Data are expressed as means ± SEM. Comparison between allergen vs diluent at various time points were made by using 2-way ANOVA with Sidaks’s multiple comparison test. Correlations were determined by using Pearson’s correlation coefficient.In vitro data were analyzed by one-way-ANOVA with Tukey’s multiple comparison test, two-tailed Student’s t test or multiplet -tests with Bonferroni correction. P values of less than .05 were considered statistically significant.