Mice
Age- and sex-matched C57BL/6 mice, C57BL/6-Foxp3GFP reporter mice,
RORγtfl/fl mice, and
Foxp3creRORγtfl/fl mice (age: 6-12
weeks) were used in our experiments. RORγtfl/fl mice
were purchased from The Jackson Laboratory (USA). C57BL/6-Foxp3GFP
reporter mice and Foxp3cre mice were provided by B.
Malissen (Université de la Méditerranée, Marseille, France) and A.
Yoshimura (Keio University, Japan), respectively. All mice were
maintained under specific pathogen-free conditions. All experiments
described in this report were performed according to the Guide for
the Care and Use of Laboratory Animals at the University of Tsukuba and
were approved by the Animal Ethics Review Committee of the University of
Tsukuba.