Figure 2. Infiltration of Tr17 cells into ankle joints after onset of CIA through high expression of CCR6
a) Lymphocytes were harvested from draining lymph nodes on 10, 42, and 56 days and from ankle joints on 42 and 56 days after immunization of CII emulsified with complete Freund’s adjuvant in C57BL/6 mice. a) The expression of RORγt in Foxp3+Treg cells was chronologically analyzed using flow cytometry. b) Bar graph shows percentage of Tr17 cells in LN and AJ on day 10, 42, and 56. c) At 10 days after CII immunization CCR6 expression on Tr17 cells, RORγt-Treg cells, and RORγt+non-Treg cells in lymph node were analyzed using flow cytometry.