3.5 Impact of physical activity on cytokine levels of polyclonally stimulated PBMCs
Asthmatic children with high PA showed elevated levels of IL-1β, IL-5, IL-6, IL-7, IL-9, IL-12p70, IL-13, IL-17A, IL23A, IL-27, TNF-α, CCL4, CCL5, CXCL8 and CXCL10 in response to stimulation with PHA, poly I:C, R848 and zymo (Fig. 4A). A significant decrease was only observed for IL-10 in PBMCs stimulated with R848 and for IL-25 after stimulation with PHA, poly I:C and zymo.
In healthy children, high PA increased the levels of IL-5, IL-7, IL-10, IL-17A, IL-33, TNF-α, CCL3, CCL4 and CXCL10 after PBMC stimulation with PHA, poly I:C, R848 and zymo (Fig. 4B). Cytokines that showed no significant difference between asthmatic and healthy subjects according to their PA level are found in Online Supplementary (S-Fig. 8 - S-Fig. 11).