3.6 Impact of TV attendance on stimulated cytokine levels of
polyclonally stimulated PBMCs
Stimulation with PHA and R848 led to higher levels of IL-10 in
asthmatics with high daily TVA (Fig. 5A). Elevated levels were also
observed for CXCL8 after stimulation with zymo. A decrease in secretion
of IL-1β, IL-6, IL-12p70, IL-13, IL-17A, IL-25, IL-33, IFNα2, IFNγ and
CXCL10 was observed in response to stimulation with PHA, poly I:C, R848
and zymo.
Daily long-time TVA in healthy children increased levels of IL-7,
IL-12p70, IL-23A, TNF-α, IFNα2, IFNγ, CCL3, CCL5 and CXCL10 in PBMC
cultures stimulated by PHA, poly I:C, R848 and zymo. A slight reduction
in IL-33 and CXCL8 levels was observed for healthy subjects with longer
TVA after stimulation with zymo and PHA, respectively. Cytokines that
showed no significant difference between asthmatic and healthy subjects
according to their daily TVA are found in Online Supplementary (S-Fig.
12 - S-Fig. 15).