Analytical sensitivity
Analytical sensitivity was measured using the limit of blank (LoB) and limit of detection (LoD) assays. As shown in Table 4 , LoB ofA. baumannii and K. pneumonia were 0.55 copies/µL and 0.15 copies/µL, respectively; LoD of A. baumannii and K. pneumonia were 0.93 copies/µL and 0.27 copies/µL, respectively. LoD served as the lowest concentration that gave positive results in the ddPCR assay.