Analytical specificity
To detect A. baumannii and K. pneumonia in blood samples,
we designed a specific primer-probe set for each strain. The specificity
test showed that all 5 A. baumannii isolates and 33 K.
pneumonia isolates from ATCC were detected by corresponding
primer-probe set, whereas the 131 negative control isolates showed no
response to the primer-probe sets (Table 4 ). These results
suggest that the primer-probe sets are specific to A. baumanniiand K. pneumonia , respectively.