Primers and probes
Primers and TaqMan MGB probes (Table 2 ) were designed using
Primer Express (Thermo Fisher Scientific, Waltham, MA, USA) and
synthesized by General Biosystems (Chuzhou, China). A ROX or Cy5
reporter was incorporated at the 5’ end of the probes, and a
nonfluorescent quencher at the 3’ end. The sensitivity and specificity
were evaluated using the sequence alignments in GenBank and the Basic
Local Alignment Search Tool on NCBI, respectively.