Each of the six Prolixibacter strains was grown anaerobically at 25°C for 30 days under an atmosphere of N₂:CO₂ (4:1) in a 50-ml serum bottle containing 20 ml of ammonium-free YSw medium or the medium supplemented with 10 mM nitrate and/or 2.8 mM ammonium. The concentrations of nitrate, nitrite, and ammonium in each culture or in an aseptic control at day 30 were determined, and those in the aseptic control were subtracted from the respective values in each culture. Data represent means and standard deviations (n = 3).

N.A.: not applicable.

Table 3. Fe⁰ corrosion and nitrate reduction to nitrite and ammonium in the cultures of Prolixibacter strains.