3.4∣Patch delivery suppressed the infiltration of eosinophils in the skin lesion
Based on our previous study, delayed reactions of midge allergy involve multiple inflammatory cell infiltration, especially eosinophils.12,19 Histological examination was conducted using skin tissues derived from mice given different doses of For t 2 DNA patches (Figure S6A). The data clearly showed that doses of 10 and 25 μg/patch markedly decreased the infiltration of eosinophils on rFor t 2-challenged abdominal skins, as shown in Figure S6B.
Taken together, our pilot study revealed that the optimal dose of For t 2 DNA vaccine was 25 μg/patch. We therefore performed the rest of the experiments with this dose.