3.4∣Patch delivery suppressed the infiltration of eosinophils in
the skin lesion
Based on our previous study, delayed reactions of midge allergy involve
multiple inflammatory cell infiltration, especially
eosinophils.12,19 Histological examination was
conducted using skin tissues derived from mice given different doses of
For t 2 DNA patches (Figure S6A). The data clearly showed that doses of
10 and 25 μg/patch markedly decreased the infiltration of eosinophils on
rFor t 2-challenged abdominal skins, as shown in Figure S6B.
Taken together, our pilot study revealed that the optimal dose of For t
2 DNA vaccine was 25 μg/patch. We therefore performed the rest of the
experiments with this dose.