2.8∣Measurement of cytokine production
Splenocytes were cultured in 24-well plates at a concentration of
1×106/mL and stimulated with various doses of rFor t 2
at 37℃ for 3-5 days. The culture supernatants were collected at each
time interval and stored at -20℃ until use in the cytokine assay. The
levels of IL-10, IL-13, and interferon-γ (IFN-γ) in the culture
supernatants of splenocytes were measured with murine ELISA kits
(eBioscience, San Diego, CA, USA) according to the instructions of the
manufacturer.