Figure 7. Compound 270 relieves the mice acute lung injury triggered by intraperitoneal injection of LPS through eliminating lung inflammation state
(A) Histological assessment of lung tissues was evaluated by HE staining. Scale bar = 200 μm, (n = 6). (B) The relative mRNA levels of pro-inflammation genes in lung tissues were quantified by RT-PCR normalized by GAPDH, (n = 6). (C) The protein expression of NF-κB and JNK signaling pathways in lung tissues was measured by western blot analysis, (n = 6). (D) Quantification of the relative protein expression shown in (C) was performed by densitometric analysis, (n = 6). The data are presented as the means ± SEM. One-tailed Student’s t-test. *P < 0.05, **P <0.01, ***P < 0.001 compared with the LPS group.
Figure 8