2.12 Determination of MPO and MDA
About 30 mg of left upper kidney and lung tissues were homogenated in cold saline solution at a ratio of 1:10 (weight:volume). Myeloperoxidase (MPO) activity is a biochemical marker for the infiltration of neutrophils and macrophages. Malondialdehyde (MDA) is an indicator of oxidant stress (Jiang et al. , 2019). MPO activity and MDA content were measured using a commercially available kit following manufacturer’s instructions (Nanjing Jiancheng Bioengineering Institute). Data were calculated as the absorbance after normalization by the protein content.