3.5 Compound 270 alleviates the excessive biosynthesis of
inflammatory cytokines induced by intraperitoneal injection of LPS in
C57BL/6J mice
Our observations had demonstrated that the anti-inflammation activity of
270 on LPS- induced macrophages in vitro. Subsequently, we further
evaluated the plausible effect of 270 in vivo. LPS, the main pathogenic
factor of sepsis, activates a cascade of inflammatory response, which
could result in damage to organs. LPS is the pivotal stimulus for the
massive production of various inflammatory mediators, which has widely
been applied to establish sepsis-associated AKI and ALI mice models
(Ju et al. , 2018;
Islam et al. , 2019). So we
determined the effect of oral administration with 270 on mice injected
intraperitoneally with LPS (10 mg/kg) for 24 h. The effect of
pretreatment with 270 on food take and body weight was firstly
determined, there were no obvious differences among the various groups
(Figure 5A). Whereas, the kidney index of mice in the LPS group was
significantly higher than that in the Veh group, and pretreatment with
270 markedly relieved the kidney index of LPS-induced mice, and no
distinct changes were discovered in the liver and lung index among the
various groups (Figure 5B). The plasma levels of TNF-α, IL-1β, IL-6 and
MCP 1 were dramatically elevated after intraperitoneal injection of LPS,
and these alterations of pro-inflammatory cytokines following LPS
challenge were remarkably ameliorated by administration with 270 (Figure
5C). Our findings reflected that 270 have anti-inflammation activity in
vivo and may exert direct effect on kidney in LPS-induced mice.